ethyl 3-(4-(methylamino)-3-nitro-N-(pyridin-2-yl)benzamido)propanoate（CAS# 429659-01-8)

Introduction

Ethyl 3-(4-(methylamino)-3-nitro-N-(pyridin-2-yl)benzoylamido)propionate, also known as MTT (Meta-Toluidine ortho-Toluenesulphonic acid triazine ester), is a chemical substance. It has the following properties:

Appearance: White solid

Solubility: Soluble in alcohols, ketones and chloroform

The main uses of MTT are as follows:

Biological research: MTT is widely used to determine cell proliferation and cell viability. It converts MTT into a blue product that dissolves in cells by reducing it, which can be used to determine cell viability.

In general, the preparation method of MTT can be carried out by the following steps:

Methylaniline is reacted with 2-nitrobenzoyl chloride to obtain 3-(4-methylaminobenzoyl)benzoyl chloride.

The product obtained in step 1 is reacted with 2-pyridone to generate 3-(4-methylaminobenzoylamido)benzoyl chloride.

The product obtained in step 2 is reacted with ethyl 3-aminopropionate to generate ethyl 3-(4-methylaminobenzoylamido)propionate.

The final product, MTT, is purified by crystallization or other methods.

Safety Information: MTT is a toxic substance, and care should be taken to use and dispose of it properly. Appropriate protective equipment such as gloves, glasses, and laboratory coatings need to be worn during operation. Avoid inhalation, skin contact, and eating. When using or disposing of waste, follow relevant safety operating procedures, and promptly dispose of and clean any inadvertent exposure. In case of any inhalation or skin contact, seek medical attention immediately.